3F4C

Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound

3F4C の概要

• エントリーDOI: 10.2210/pdb3f4c/pdb
• 関連するPDBエントリー: 3F4D
• 分子名称: Organophosphorus hydrolase, COBALT (II) ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: alpha-beta barrel, amidohydrolase, binuclear metal enzyme, glycerol-bound, hydrolase
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74972.73

構造登録者

Hawwa, R.,Aikens, J.,Turner, R.J.,Santarsiero, B.,Mesecar, A. (登録日: 2008-10-31, 公開日: 2009-09-15, 最終更新日: 2023-11-15)

主引用文献

Hawwa, R.,Aikens, J.,Turner, R.J.,Santarsiero, B.D.,Mesecar, A.D.
Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.
Arch.Biochem.Biophys., 488:109-120, 2009

PubMed Abstract: A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100 degrees C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60 degrees C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.

PubMed: 19615330
DOI: 10.1016/j.abb.2009.06.005

実験手法

X-RAY DIFFRACTION (2.07 Å)