3F2C

DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn

3F2C の概要

• エントリーDOI: 10.2210/pdb3f2c/pdb
• 関連するPDBエントリー: 3F2B 3F2D
• 分子名称: GEOBACILLUS KAUSTOPHILUS DNA POLC, 5'-D(*DCP*DAP*DGP*DTP*DGP*DAP*DGP*DAP*DCP*DGP*DGP*DGP*DCP*DAP*DAP*DCP*DC)-3', 5'-D(*DAP*DTP*DAP*DAP*DCP*DGP*DGP*DTP*DTP*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DTP*DCP*DAP*DCP*DTP*DG)-3', ... (8 entities in total)
• 機能のキーワード: dna polymerase c, dna polymerase iii, ternary complex, protein-dna complex, replicative polymerase, gram-positive, transferase-dna complex, transferase/dna
• 由来する生物種: Geobacillus kaustophilus; 詳細
• 細胞内の位置: Cytoplasm : Q5L0J3
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 130031.72

構造登録者

Davies, D.R.,Evans, R.J.,Bullard, J.M.,Christensen, J.,Green, L.S.,Guiles, J.W.,Ribble, W.K.,Janjic, N.,Jarvis, T.C. (登録日: 2008-10-29, 公開日: 2009-01-20, 最終更新日: 2023-09-06)

主引用文献

Evans, R.J.,Davies, D.R.,Bullard, J.M.,Christensen, J.,Green, L.S.,Guiles, J.W.,Pata, J.D.,Ribble, W.K.,Janjic, N.,Jarvis, T.C.
Structure of PolC reveals unique DNA binding and fidelity determinants.
Proc.Natl.Acad.Sci.USA, 105:20695-20700, 2008

PubMed Abstract: PolC is the polymerase responsible for genome duplication in many Gram-positive bacteria and represents an attractive target for antibacterial development. We have determined the 2.4-A resolution crystal structure of Geobacillus kaustophilus PolC in a ternary complex with DNA and dGTP. The structure reveals nascent base pair interactions that lead to highly accurate nucleotide incorporation. A unique beta-strand motif in the PolC thumb domain contacts the minor groove, allowing replication errors to be sensed up to 8 nt upstream of the active site. PolC exhibits the potential for large-scale conformational flexibility, which could encompass the catalytic residues. The structure suggests a mechanism by which the active site can communicate with the rest of the replisome to trigger proofreading after nucleotide misincorporation, leading to an integrated model for controlling the dynamic switch between replicative and repair polymerases. This ternary complex of a cellular replicative polymerase affords insights into polymerase fidelity, evolution, and structural diversity.

PubMed: 19106298
DOI: 10.1073/pnas.0809989106

実験手法

X-RAY DIFFRACTION (2.5 Å)