3EZG

Crystal structure of E18Q DJ-1 with oxidized C106

3EZG の概要

• エントリーDOI: 10.2210/pdb3ezg/pdb
• 関連するPDBエントリー: 1SOA 3CY6
• 分子名称: Protein DJ-1 (2 entities in total)
• 機能のキーワード: cysteine oxidation, sulfinic acid, chaperone, disease mutation, nucleus, oncogene, oxidation, parkinson disease, phosphoprotein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Lipid-anchor: Q99497
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20881.07

構造登録者

Lakshminarasimhan, M.,Wilson, M.A. (登録日: 2008-10-22, 公開日: 2008-12-30, 最終更新日: 2024-10-30)

主引用文献

Blackinton, J.,Lakshminarasimhan, M.,Thomas, K.J.,Ahmad, R.,Greggio, E.,Raza, A.S.,Cookson, M.R.,Wilson, M.A.
Formation of a stabilized cysteine sulfinic acid is critical for the mitochondrial function of the parkinsonism protein DJ-1.
J.Biol.Chem., 284:6476-6485, 2009

PubMed Abstract: The formation of cysteine-sulfinic acid has recently become appreciated as a modification that links protein function to cellular oxidative status. Human DJ-1, a protein associated with inherited parkinsonism, readily forms cysteine-sulfinic acid at a conserved cysteine residue (Cys106 in human DJ-1). Mutation of Cys106 causes the protein to lose its normal protective function in cell culture and model organisms. However, it is unknown whether the loss of DJ-1 protective function in these mutants is due to the absence of Cys106 oxidation or the absence of the cysteine residue itself. To address this question, we designed a series of substitutions at a proximal glutamic acid residue (Glu18) in human DJ-1 that alter the oxidative propensity of Cys106 through changes in hydrogen bonding. We show that two mutations, E18N and E18Q, allow Cys106 to be oxidized to Cys106-sulfinic acid under mild conditions. In contrast, the E18D mutation stabilizes a cysteine-sulfenic acid that is readily reduced to the thiol in solution and in vivo. We show that E18N and E18Q can both partially substitute for wild-type DJ-1 using mitochondrial fission and cell viability assays. In contrast, the oxidatively impaired E18D mutant behaves as an inactive C106A mutant and fails to protect cells. We therefore conclude that formation of Cys106-sulfinic acid is a key modification that regulates the protective function of DJ-1.

PubMed: 19124468
DOI: 10.1074/jbc.M806599200

実験手法

X-RAY DIFFRACTION (1.15 Å)