3EXD

Sulfur-SAD phased HEWL Crystal

3EXD の概要

• エントリーDOI: 10.2210/pdb3exd/pdb
• 分子名称: Lysozyme C (2 entities in total)
• 機能のキーワード: hewl, sulfur, sad phasing, allergen, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14331.16

構造登録者

Nascimento, A.S.,Liberato, M.V.,Polikarpov, I. (登録日: 2008-10-16, 公開日: 2008-10-28, 最終更新日: 2024-10-30)

主引用文献

Guimaraes, B.G.,Sanfelici, L.,Neuenschwander, R.T.,Rodrigues, F.,Grizolli, W.C.,Raulik, M.A.,Piton, J.R.,Meyer, B.C.,Nascimento, A.S.,Polikarpov, I.
The MX2 macromolecular crystallography beamline: a wiggler X-ray source at the LNLS.
J.Synchrotron Radiat., 16:69-75, 2009

PubMed Abstract: The Brazilian Synchrotron Light Laboratory [Laboratório Nacional de Luz Síncrotron (LNLS), Campinas, SP, Brazil] is the first commissioned synchrotron light source in the southern hemisphere. The first wiggler macromolecular crystallography beamline (MX2) at the LNLS has been recently constructed and brought into operation. Here the technical design, experimental set-up, parameters of the beamline and the first experimental results obtained at MX2 are described. The beamline operates on a 2.0 T hybrid 30-pole wiggler, and its optical layout includes collimating mirror, Si(111) double-crystal monochromator and toroidal bendable mirror. The measured flux density at the sample position at 8.7 eV reaches 4.8 x 10(11) photons s(-1) mm(-2) (100 mA)(-1). The beamline is equipped with a MarResearch Desktop Beamline Goniostat (MarDTB) and 3 x 3 MarMosaic225 CCD detector, and is controlled by a customized version of the Blu-Ice software. A description of the first X-ray diffraction data sets collected at the MX2 LNLS beamline and used for macromolecular crystal structure solution is also provided.

PubMed: 19096177
DOI: 10.1107/S0909049508034870

実験手法

X-RAY DIFFRACTION (1.49 Å)