3EWG

Crystal structure of the N-terminal domain of NusG (NGN) from Methanocaldococcus jannaschii

3EWG の概要

• エントリーDOI: 10.2210/pdb3ewg/pdb
• 分子名称: Putative transcription antitermination protein nusG, 1,4-DIETHYLENE DIOXIDE (3 entities in total)
• 機能のキーワード: alpha/beta structure, antiparallel beta sheet, flanked alpha helices, transcription
• 由来する生物種: Methanocaldococcus jannaschii (Methanococcus jannaschii)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10329.98

構造登録者

Zhou, H.H.,Liu, Q.,Gao, Y.X.,Teng, M.K.,Niu, L.W. (登録日: 2008-10-15, 公開日: 2009-06-16, 最終更新日: 2023-11-01)

主引用文献

Zhou, H.H.,Liu, Q.,Gao, Y.X.,Teng, M.K.,Niu, L.W.
Crystal structure of NusG N-terminal (NGN) domain from Methanocaldococcus jannaschii and its interaction with rpoE''
Proteins, 76:787-793, 2009

PubMed Abstract: Transcription in archaea employs a eukaryotic-type transcription apparatus but uses bacterial-type transcription factors. NusG is one of the few archaeal transcription factors whose orthologs are essential in both bacteria and eukaryotes. Archaeal NusG is composed of only an NusG N-terminal (NGN) domain and a KOW domain, which is similar to bacterial NusG but not to the eukaryotic ortholog, Spt5. However, archaeal NusG was confirmed recently to form a complex with rpoE'' that was similar to the Spt5-Spt4 complex. Thus, archaeal NusG presents hybrid features of Spt5 and bacterial NusG. Here we report the crystal structure of NGN from the archaea Methanocaldococcus jannaschii (MjNGN). MjNGN folds to an alpha-beta-alpha sandwich without the appendant domain of bacterial NGNs, and forms a unique homodimer in crystal and solution. MjNGN alone was found to be sufficient for rpoE'' binding and an MjNGN-rpoE'' model has been constructed by rigid docking.

PubMed: 19475703
DOI: 10.1002/prot.22465

実験手法

X-RAY DIFFRACTION (2.04 Å)