3EWE

Crystal Structure of the Nup85/Seh1 Complex

3EWE の概要

• エントリーDOI: 10.2210/pdb3ewe/pdb
• 分子名称: Nucleoporin SEH1, Nucleoporin NUP85 (2 entities in total)
• 機能のキーワード: nucleoporin, nuclear pore complex, mrna transport, nucleus, protein transport, translocation, cell membrane, membrane, phosphoprotein, transport, wd repeat, membrane protein, structural protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast,yeast); 詳細
• 細胞内の位置: Nucleus, nuclear pore complex: P53011 P46673
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 206758.32

構造登録者

Brohawn, S.G.,Leksa, N.C.,Rajashankar, K.R.,Schwartz, T.U. (登録日: 2008-10-14, 公開日: 2008-11-11, 最終更新日: 2024-10-09)

主引用文献

Brohawn, S.G.,Leksa, N.C.,Spear, E.D.,Rajashankar, K.R.,Schwartz, T.U.
Structural evidence for common ancestry of the nuclear pore complex and vesicle coats.
Science, 322:1369-1373, 2008

PubMed Abstract: Nuclear pore complexes (NPCs) facilitate nucleocytoplasmic transport. These massive assemblies comprise an eightfold symmetric scaffold of architectural proteins and central-channel phenylalanine-glycine-repeat proteins forming the transport barrier. We determined the nucleoporin 85 (Nup85)*Seh1 structure, a module in the heptameric Nup84 complex, at 3.5 angstroms resolution. Structural, biochemical, and genetic analyses position the Nup84 complex in two peripheral NPC rings. We establish a conserved tripartite element, the ancestral coatomer element ACE1, that reoccurs in several nucleoporins and vesicle coat proteins, providing structural evidence of coevolution from a common ancestor. We identified interactions that define the organization of the Nup84 complex on the basis of comparison with vesicle coats and confirmed the sites by mutagenesis. We propose that the NPC scaffold, like vesicle coats, is composed of polygons with vertices and edges forming a membrane-proximal lattice that provides docking sites for additional nucleoporins.

PubMed: 18974315
DOI: 10.1126/science.1165886

実験手法

X-RAY DIFFRACTION (3.5 Å)