3ERX

High-resolution structure of Paracoccus pantotrophus pseudoazurin

3ERX の概要

• エントリーDOI: 10.2210/pdb3erx/pdb
• 関連するPDBエントリー: 1ADW
• 分子名称: Pseudoazurin, COPPER (II) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: pseudoazurin, copper protein, paracoccus, high-resolution, electron transport, metal-binding, transport
• 由来する生物種: Paracoccus pantotrophus (Thiosphaera pantotropha)
• 細胞内の位置: Periplasm: P80401
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27131.74

構造登録者

Najmudin, S.,Pauleta, S.R.,Moura, I.,Romao, M.J. (登録日: 2008-10-03, 公開日: 2009-10-13, 最終更新日: 2023-09-06)

主引用文献

Najmudin, S.,Pauleta, S.R.,Moura, I.,Romao, M.J.
The 1.4 A resolution structure of Paracoccus pantotrophus pseudoazurin.
Acta Crystallogr.,Sect.F, 66:627-635, 2010

PubMed Abstract: Pseudoazurins are small type 1 copper proteins that are involved in the flow of electrons between various electron donors and acceptors in the bacterial periplasm, mostly under denitrifying conditions. The previously determined structure of Paracoccus pantotrophus pseudoazurin in the oxidized form was improved to a nominal resolution of 1.4 A, with R and R(free) values of 0.188 and 0.206, respectively. This high-resolution structure makes it possible to analyze the interactions between the monomers and the solvent structure in detail. Analysis of the high-resolution structure revealed the structural regions that are responsible for monomer-monomer recognition during dimer formation and for protein-protein interaction and that are important for partner recognition. The pseudoazurin structure was compared with other structures of various type 1 copper proteins and these were grouped into families according to similarities in their secondary structure; this may be useful in the annotation of copper proteins in newly sequenced genomes and in the identification of novel copper proteins.

PubMed: 20516588
DOI: 10.1107/S1744309110013989

実験手法

X-RAY DIFFRACTION (1.25 Å)