3ENR

ZINC-CALCIUM CONCANAVALIN A AT PH 6.15

3ENR の概要

• エントリーDOI: 10.2210/pdb3enr/pdb
• 分子名称: Concanavalin-A, ZINC ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: concanavalin a, lectin, metal binding, ph
• 由来する生物種: Canavalia ensiformis (Jack bean); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51586.56

構造登録者

Bouckaert, J.,Loris, R.,Wyns, L. (登録日: 1999-02-11, 公開日: 2000-09-18, 最終更新日: 2023-09-06)

主引用文献

Bouckaert, J.,Loris, R.,Wyns, L.
Zinc/calcium- and cadmium/cadmium-substituted concanavalin A: interplay of metal binding, pH and molecular packing.
Acta Crystallogr.,Sect.D, 56:1569-1576, 2000

PubMed Abstract: The crystal structures of cadmium/cadmium and zinc/calcium concanavalin A (con A) at pH 5.0 and pH 6.15, respectively, were determined. The structure of cadmium/cadmium con A confirms that the secondary Cd(2+)-binding site S3 is empty at pH 5. The metal-binding sites S1 and S2 are only very slightly affected by the substitution with cadmium. On the other hand, S1 and S2 and most of the protein surface of zinc/calcium con A at pH 6.15 differ from other fully metal-bound and carbohydrate-free structures. Most of these structural differences at the protein surface are a result of the interplay between metal binding, protonation and crystal packing. This interplay is expressed by relative rotations and translations of the con A units in alternative crystal packings and participation in space-group conversions inside crystals in situ. The particular crystal packing of zinc/calcium con A creates a novel zinc-binding site S4. The Zn(2+) ion in S4 ligates two aspartates from one tetramer and a histidine from a symmetry-related tetramer.

PubMed: 11092923
DOI: 10.1107/S0907444900013342

実験手法

X-RAY DIFFRACTION (2.4 Å)