3ELN

A Putative Fe2+-bound Persulfenate Intermediate in Cysteine Dioxygenase

3ELN の概要

• エントリーDOI: 10.2210/pdb3eln/pdb
• 関連するPDBエントリー: 2b5h 2gh2
• 分子名称: Cysteine dioxygenase type 1, FE (II) ION, S-HYDROPEROXYCYSTEINE, ... (4 entities in total)
• 機能のキーワード: peroxysulfenate, non-heme dioxygenases, fe2+ metalloenzyme, cysteine, taurine, thioether, dioxygenase, iron, metal-binding, oxidoreductase, phosphoprotein, thioether bond
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23267.89

構造登録者

Simmons, C.R.,Stipanuk, M.H.,Karplus, P.A. (登録日: 2008-09-22, 公開日: 2008-10-21, 最終更新日: 2024-02-21)

主引用文献

Simmons, C.R.,Krishnamoorthy, K.,Granett, S.L.,Schuller, D.J.,Dominy, J.E.,Begley, T.P.,Stipanuk, M.H.,Karplus, P.A.
A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.
Biochemistry, 47:11390-11392, 2008

PubMed Abstract: The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediate trapped in its active site pocket. The complex raises the possibility that, distinct from known dioxygenases and proposed CDO mechanisms, the Fe-proximal oxygen atom may be involved in the primary oxidation event yielding a unique three-membered Fe-S-O cyclic intermediate. A nonpolar environment of the distal oxygen would facilitate isomerization of the persulfenate to the sulfinate product.

PubMed: 18847220
DOI: 10.1021/bi801546n

実験手法

X-RAY DIFFRACTION (1.42 Å)