3EIV

Crystal Structure of Single-stranded DNA-binding protein from Streptomyces coelicolor

3EIV の概要

• エントリーDOI: 10.2210/pdb3eiv/pdb
• 分子名称: Single-stranded DNA-binding protein 2 (2 entities in total)
• 機能のキーワード: single-stranded dna-binding protein, streptomyces coelicolor, dna damage, dna repair, dna replication, dna-binding, phosphoprotein, dna binding protein
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 79702.12

構造登録者

Luic, M.,Stefanic, Z.,Vujaklija, D. (登録日: 2008-09-17, 公開日: 2009-09-08, 最終更新日: 2023-08-30)

主引用文献

Stefanic, Z.,Vujaklija, D.,Luic, M.
Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor.
Acta Crystallogr.,Sect.D, 65:974-979, 2009

PubMed Abstract: The crystal structure of the single-stranded DNA-binding protein (SSB) from Streptomyces coelicolor, a filamentous soil bacterium with a complex life cycle and a linear chromosome, has been solved and refined at 2.1 A resolution. The three-dimensional structure shows a common conserved central OB-fold that is found in all structurally determined SSB proteins. However, it shows variations in quaternary structure that have previously only been found in mycobacterial SSBs. The strand involved in the clamp mechanism characteristic of this type of quaternary structure leads to higher stability of the homotetramer. To the best of our knowledge, this is the first X-ray structure of an SSB protein from a member of the genus Streptomyces and it was predicted to be the most stable of the structurally characterized bacterial or human mitochondrial SSBs.

PubMed: 19690375
DOI: 10.1107/S0907444909023634

実験手法

X-RAY DIFFRACTION (2.141 Å)