3EIL

Structure of B-DNA d(CGTTAATTAACG)2 in the presence of Manganese

Summary for 3EIL

• Entry DOI: 10.2210/pdb3eil/pdb
• Descriptor: 5'-D(*DCP*DGP*DTP*DTP*DAP*DAP*DTP*DTP*DAP*DAP*DCP*DG)-3', MANGANESE (II) ION (3 entities in total)
• Functional Keywords: b-dna, manganese, dna
• Total number of polymer chains: 6
• Total formula weight: 22353.06

Authors

Millonig, H.,Pous, J.,Campos, L.J.,Subirana, J.A. (deposition date: 2008-09-16, release date: 2009-06-02, Last modification date: 2023-08-30)

Primary citation

Millonig, H.,Pous, J.,Gouyette, C.,Subirana, J.A.,Campos, J.L.
The interaction of manganese ions with DNA
J.Inorg.Biochem., 103:876-880, 2009

PubMed Abstract: We present the structure of the duplex formed by a fragment of auto-complementary DNA with the sequence d(CGTTAATTAACG). The structure was determined by X-ray crystallography. Up to date it is the first structure presenting the interaction between a DNA oligonucleotide and manganese ions. The presence of Mn2+ creates bonds among the N7 atom of guanines and phosphates. These bonds stabilize and determine the crystallographic network in a P3(2) space group, unusual in oligonucleotide crystals. The crystal structure observed is compared with those found in the presence of Mg2+, Ca2+ and Ni2+, which show different kinds of interactions. The double helices show end-to-end interactions, in a manner that the terminal guanines interact with the minor groove of the neighboring duplex, while the terminal cytosines are disordered. We have chosen this sequence since it contains a TTAA repeat. Such repeats are very rare in all genomes. We suggest that this sequence may be very susceptible to the formation of closely spaced thymine dimers.

PubMed: 19375803
DOI: 10.1016/j.jinorgbio.2009.03.004

Experimental method

X-RAY DIFFRACTION (2.6 Å)