3EII

Zinc-bound glycoside hydrolase 61 E from Thielavia terrestris

3EII の概要

• エントリーDOI: 10.2210/pdb3eii/pdb
• 関連するPDBエントリー: 3EJA
• 分子名称: protein GH61E, ZINC ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: beta sandwich, metal binding site, fibronectin type iii, unknown function
• 由来する生物種: Thielavia terrestris
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92797.85

構造登録者

Salbo, R.,Welner, D.,Lo Leggio, L.,Harris, P.,McFarland, K. (登録日: 2008-09-16, 公開日: 2009-10-27, 最終更新日: 2024-10-30)

主引用文献

Harris, P.V.,Welner, D.,McFarland, K.C.,Re, E.,Navarro Poulsen, J.C.,Brown, K.,Salbo, R.,Ding, H.,Vlasenko, E.,Merino, S.,Xu, F.,Cherry, J.,Larsen, S.,Lo Leggio, L.
Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family.
Biochemistry, 49:3305-3316, 2010

PubMed Abstract: Currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. Among the many families of glycoside hydrolases that catalyze cellulose and hemicellulose hydrolysis, few are more enigmatic than family 61 (GH61), originally classified based on measurement of very weak endo-1,4-beta-d-glucanase activity in one family member. Here we show that certain GH61 proteins lack measurable hydrolytic activity by themselves but in the presence of various divalent metal ions can significantly reduce the total protein loading required to hydrolyze lignocellulosic biomass. We also solved the structure of one highly active GH61 protein and find that it is devoid of conserved, closely juxtaposed acidic side chains that could serve as general proton donor and nucleophile/base in a canonical hydrolytic reaction, and we conclude that the GH61 proteins are unlikely to be glycoside hydrolases. Structure-based mutagenesis shows the importance of several conserved residues for GH61 function. By incorporating the gene for one GH61 protein into a commercial Trichoderma reesei strain producing high levels of cellulolytic enzymes, we are able to reduce by 2-fold the total protein loading (and hence the cost) required to hydrolyze lignocellulosic biomass.

PubMed: 20230050
DOI: 10.1021/bi100009p

実験手法

X-RAY DIFFRACTION (2.25 Å)