3EF2

Structure of the Marasmius oreades mushroom lectin (MOA) in complex with Galalpha(1,3)[Fucalpha(1,2)]Gal and Calcium.

3EF2 の概要

• エントリーDOI: 10.2210/pdb3ef2/pdb
• 分子名称: Agglutinin, alpha-L-fucopyranose-(1-2)-[alpha-D-galactopyranose-(1-3)]beta-D-galactopyranose, alpha-L-fucopyranose-(1-2)-[alpha-D-galactopyranose-(1-3)]alpha-D-galactopyranose, ... (6 entities in total)
• 機能のキーワード: lectin, beta-trefoil, calcium-binding, carbohydrate-binding, sugar-binding, sugar binding protein
• 由来する生物種: Marasmius oreades
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 136245.51

構造登録者

Grahn, E.M.,Goldstein, I.J.,Krengel, U. (登録日: 2008-09-08, 公開日: 2009-06-30, 最終更新日: 2023-11-15)

主引用文献

Grahn, E.M.,Winter, H.C.,Tateno, H.,Goldstein, I.J.,Krengel, U.
Structural Characterization of a Lectin from the Mushroom Marasmius oreades in Complex with the Blood Group B Trisaccharide and Calcium.
J.Mol.Biol., 390:457-466, 2009

PubMed Abstract: MOA (Marasmius oreades agglutinin), a lectin isolated from fruiting bodies of the mushroom M. oreades, specifically binds nonreducing terminal Galalpha(1,3)Gal carbohydrates, such as that which occurs in the xenotransplantation epitope Galalpha(1,3)Galbeta(1,4)GlcNAc and the branched blood group B determinant Galalpha(1,3)[Fucalpha(1,2)]Gal. Here, we present the crystal structure of MOA in complex with the blood group B trisaccharide solved at 1.8 A resolution. To our knowledge, this is the first blood-group-B-specific structure reported in complex with a blood group B determinant. The carbohydrate ligand binds to all three binding sites of the N-terminal beta-trefoil domain. Also, in this work, Ca(2+) was included in the crystals, and binding of Ca(2+) to the MOA homodimer altered the conformation of the C-terminal domain by opening up the cleft containing a putative catalytic site.

PubMed: 19426740
DOI: 10.1016/j.jmb.2009.04.074

実験手法

X-RAY DIFFRACTION (1.8 Å)