3EF0

The Structure of Fcp1, an essential RNA polymerase II CTD phosphatase

3EF0 の概要

• エントリーDOI: 10.2210/pdb3ef0/pdb
• 関連するPDBエントリー: 3EF1
• 分子名称: RNA polymerase II subunit A C-terminal domain phosphatase, TETRAFLUOROALUMINATE ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: phosphatase, ctd, fcph, brct, hydrolase, alf4, transition state analog, cobalt, magnesium, manganese, metal-binding, nucleus, protein phosphatase
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast); 詳細
• 細胞内の位置: Nucleus : Q9P376
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42510.71

構造登録者

Ghosh, A.,Lima, C.D. (登録日: 2008-09-07, 公開日: 2008-12-02, 最終更新日: 2024-04-03)

主引用文献

Ghosh, A.,Shuman, S.,Lima, C.D.
The structure of Fcp1, an essential RNA polymerase II CTD phosphatase.
Mol.Cell, 32:478-490, 2008

PubMed Abstract: Kinases and phosphatases regulate mRNA synthesis and processing by phosphorylating and dephosphorylating the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. Fcp1 is an essential CTD phosphatase that preferentially hydrolyzes Ser2-PO(4) of the tandem YSPTSPS CTD heptad array. Fcp1 crystal structures were captured at two stages of the reaction pathway: a Mg-BeF(3) complex that mimics the aspartylphosphate intermediate and a Mg-AlF(4)(-) complex that mimics the transition state of the hydrolysis step. Fcp1 is a Y-shaped protein composed of an acylphosphatase domain located at the base of a deep canyon formed by flanking modules that are missing from the small CTD phosphatase (SCP) clade: an Fcp1-specific helical domain and a C-terminal BRCA1 C-terminal (BRCT) domain. The structure and mutational analysis reveals that Fcp1 and Scp1 (a Ser5-selective phosphatase) adopt different CTD-binding modes; we surmise the CTD threads through the Fcp1 canyon to access the active site.

PubMed: 19026779
DOI: 10.1016/j.molcel.2008.09.021

実験手法

X-RAY DIFFRACTION (2.1 Å)