3EBE
Crystal structure of xenopus laevis replication initiation factor MCM10 internal domain
Summary for 3EBE
| Entry DOI | 10.2210/pdb3ebe/pdb |
| Descriptor | Protein MCM10 homolog, ZINC ION (3 entities in total) |
| Functional Keywords | ob-fold, zinc finger, ccch, dna replication, metal-binding, nucleus, zinc-finger, replication |
| Biological source | Xenopus laevis (clawed frog,common platanna,platanna) |
| Cellular location | Nucleus: Q5EAW4 |
| Total number of polymer chains | 3 |
| Total formula weight | 68296.18 |
| Authors | Warren, E.M.,Eichman, B.F. (deposition date: 2008-08-27, release date: 2008-12-09, Last modification date: 2024-02-21) |
| Primary citation | Warren, E.M.,Vaithiyalingam, S.,Haworth, J.,Greer, B.,Bielinsky, A.K.,Chazin, W.J.,Eichman, B.F. Structural basis for DNA binding by replication initiator mcm10. Structure, 16:1892-1901, 2008 Cited by PubMed Abstract: Mcm10 is an essential eukaryotic DNA replication protein required for assembly and progression of the replication fork. The highly conserved internal domain (Mcm10-ID) has been shown to physically interact with single-stranded (ss) DNA, DNA polymerase alpha, and proliferating cell nuclear antigen (PCNA). The crystal structure of Xenopus laevis Mcm10-ID presented here reveals a DNA binding architecture composed of an oligonucleotide/oligosaccharide-fold followed in tandem by a variant and highly basic zinc finger. NMR chemical shift perturbation and mutational studies of DNA binding activity in vitro reveal how Mcm10 uses this unique surface to engage ssDNA. Corresponding mutations in Saccharomyces cerevisiae result in increased sensitivity to replication stress, demonstrating the functional importance of DNA binding by this region of Mcm10 to replication. In addition, mapping Mcm10 mutations known to disrupt PCNA, polymerase alpha, and DNA interactions onto the crystal structure provides insight into how Mcm10 might coordinate protein and DNA binding within the replisome. PubMed: 19081065DOI: 10.1016/j.str.2008.10.005 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
Download full validation report
