3EBE

Crystal structure of xenopus laevis replication initiation factor MCM10 internal domain

3EBE の概要

• エントリーDOI: 10.2210/pdb3ebe/pdb
• 分子名称: Protein MCM10 homolog, ZINC ION (3 entities in total)
• 機能のキーワード: ob-fold, zinc finger, ccch, dna replication, metal-binding, nucleus, zinc-finger, replication
• 由来する生物種: Xenopus laevis (clawed frog,common platanna,platanna)
• 細胞内の位置: Nucleus: Q5EAW4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 68296.18

構造登録者

Warren, E.M.,Eichman, B.F. (登録日: 2008-08-27, 公開日: 2008-12-09, 最終更新日: 2024-02-21)

主引用文献

Warren, E.M.,Vaithiyalingam, S.,Haworth, J.,Greer, B.,Bielinsky, A.K.,Chazin, W.J.,Eichman, B.F.
Structural basis for DNA binding by replication initiator mcm10.
Structure, 16:1892-1901, 2008

PubMed Abstract: Mcm10 is an essential eukaryotic DNA replication protein required for assembly and progression of the replication fork. The highly conserved internal domain (Mcm10-ID) has been shown to physically interact with single-stranded (ss) DNA, DNA polymerase alpha, and proliferating cell nuclear antigen (PCNA). The crystal structure of Xenopus laevis Mcm10-ID presented here reveals a DNA binding architecture composed of an oligonucleotide/oligosaccharide-fold followed in tandem by a variant and highly basic zinc finger. NMR chemical shift perturbation and mutational studies of DNA binding activity in vitro reveal how Mcm10 uses this unique surface to engage ssDNA. Corresponding mutations in Saccharomyces cerevisiae result in increased sensitivity to replication stress, demonstrating the functional importance of DNA binding by this region of Mcm10 to replication. In addition, mapping Mcm10 mutations known to disrupt PCNA, polymerase alpha, and DNA interactions onto the crystal structure provides insight into how Mcm10 might coordinate protein and DNA binding within the replisome.

PubMed: 19081065
DOI: 10.1016/j.str.2008.10.005

実験手法

X-RAY DIFFRACTION (2.3 Å)