3E6H

MHC CLASS I H-2Dd heavy chain complexed with Beta-2 Microglobulin and a variant peptide, PI10, from the human immunodeficiency virus (BaL) envelope glycoprotein 120

3E6H の概要

• エントリーDOI: 10.2210/pdb3e6h/pdb
• 関連するPDBエントリー: 1DDH
• 分子名称: H-2 class I histocompatibility antigen, D-D alpha chain, Putative uncharacterized protein, Envelope glycoprotein 10-residue peptide, ... (4 entities in total)
• 機能のキーワード: complex (histocompatibility-antigen), glycoprotein, immune response, membrane, mhc i, phosphoprotein, transmembrane, immunoglobulin domain, secreted, envelope protein, immune system
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01900
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44994.57

構造登録者

Wang, R.,Natarajan, K.,Robinson, H.,Margulies, D.H. (登録日: 2008-08-15, 公開日: 2009-08-18, 最終更新日: 2024-11-13)

主引用文献

Honda, M.,Wang, R.,Kong, W.P.,Kanekiyo, M.,Akahata, W.,Xu, L.,Matsuo, K.,Natarajan, K.,Robinson, H.,Asher, T.E.,Price, D.A.,Douek, D.C.,Margulies, D.H.,Nabel, G.J.
Different vaccine vectors delivering the same antigen elicit CD8+ T cell responses with distinct clonotype and epitope specificity
J.Immunol., 183:2425-2434, 2009

PubMed Abstract: Prime-boost immunization with gene-based vectors has been developed to generate more effective vaccines for AIDS, malaria, and tuberculosis. Although these vectors elicit potent T cell responses, the mechanisms by which they stimulate immunity are not well understood. In this study, we show that immunization by a single gene product, HIV-1 envelope, with alternative vector combinations elicits CD8(+) cells with different fine specificities and kinetics of mobilization. Vaccine-induced CD8(+) T cells recognized overlapping third V region loop peptides. Unexpectedly, two anchor variants bound H-2D(d) better than the native sequences, and clones with distinct specificities were elicited by alternative vectors. X-ray crystallography revealed major differences in solvent exposure of MHC-bound peptide epitopes, suggesting that processed HIV-1 envelope gave rise to MHC-I/peptide conformations recognized by distinct CD8(+) T cell populations. These findings suggest that different gene-based vectors generate peptides with alternative conformations within MHC-I that elicit distinct T cell responses after vaccination.

PubMed: 19620307
DOI: 10.4049/jimmunol.0900581

実験手法

X-RAY DIFFRACTION (2.1 Å)