3E6C
CprK OCPA DNA Complex
3E6C の概要
エントリーDOI | 10.2210/pdb3e6c/pdb |
関連するPDBエントリー | 3E5Q 3E5U 3E6B 3E6D 3E6X |
分子名称 | Cyclic nucleotide-binding protein, DNA (5'-D(P*DGP*DCP*DAP*DTP*DTP*DAP*DAP*DCP*DAP*DTP*DGP*DCP*DC)-3'), DNA (5'-D(P*DGP*DGP*DCP*DAP*DTP*DGP*DTP*DTP*DAP*DAP*DTP*DGP*DC)-3'), ... (5 entities in total) |
機能のキーワード | cprk, halorespiration, transcriptional regulator, transcription regulation-dna complex, transcription regulation/dna |
由来する生物種 | Desulfitobacterium hafniense |
タンパク質・核酸の鎖数 | 3 |
化学式量合計 | 36508.42 |
構造登録者 | |
主引用文献 | Levy, C.,Pike, K.,Heyes, D.J.,Joyce, M.G.,Gabor, K.,Smidt, H.,van der Oost, J.,Leys, D. Molecular basis of halorespiration control by CprK, a CRP-FNR type transcriptional regulator Mol.Microbiol., 70:151-167, 2008 Cited by PubMed Abstract: Certain bacteria are able to conserve energy via the reductive dehalogenation of halo-organic compounds in a respiration-type metabolism. The transcriptional regulator CprK from Desulfitobacterium spp. induces expression of halorespiratory genes upon binding of o-chlorophenol ligands and is reversibly inactivated by oxygen through disulphide bond formation. We report crystal structures of D. hafniense CprK in the ligand-free (both oxidation states), ligand-bound (reduced) and DNA-bound states, making it the first member of the widespread CRP-FNR superfamily for which a complete structural description of both redox-dependent and allosteric molecular rearrangements is available. In conjunction with kinetic and thermodynamic ligand binding studies, we provide a model for the allosteric mechanisms underpinning transcriptional control. Amino acids that play a key role in this mechanism are not conserved in functionally distinct CRP-FNR members. This suggests that, despite significant structural homology, distinct allosteric mechanisms are used, enabling this protein family to control a very wide range of processes. PubMed: 18717788DOI: 10.1111/j.1365-2958.2008.06399.x 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (1.8 Å) |
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