3E4H

Crystal structure of the cyclotide varv F

3E4H の概要

• エントリーDOI: 10.2210/pdb3e4h/pdb
• 分子名称: Varv peptide F,Varv peptide F (2 entities in total)
• 機能のキーワード: cyclotide, circular proteins, cystine knot, cyclization, plant protein, knottin, plant defense
• 由来する生物種: Viola arvensis (European field pansy); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2984.43

構造登録者

Hu, S.H. (登録日: 2008-08-11, 公開日: 2009-02-10, 最終更新日: 2024-10-16)

主引用文献

Wang, C.K.,Hu, S.H.,Martin, J.L.,Hajdu, J.,Bohlin, L.,Claeson, P.,Rosengren, K.J.,Tang, J.,Tan, N.H.,Craik, D.J.
Combined X-ray and NMR Analysis of the Stability of the Cyclotide Cystine Knot Fold That Underpins Its Insecticidal Activity and Potential Use as a Drug Scaffold
J.Biol.Chem., 284:10672-10683, 2009

PubMed Abstract: Cyclotides are a family of plant defense proteins that are highly resistant to adverse chemical, thermal, and enzymatic treatment. Here, we present the first crystal structure of a cyclotide, varv F, from the European field pansy, Viola arvensis, determined at a resolution of 1.8 A. The solution state NMR structure was also determined and, combined with measurements of biophysical parameters for several cyclotides, provided an insight into the structural features that account for the remarkable stability of the cyclotide family. The x-ray data confirm the cystine knot topology and the circular backbone, and delineate a conserved network of hydrogen bonds that contribute to the stability of the cyclotide fold. The structural role of a highly conserved Glu residue that has been shown to regulate cyclotide function was also determined, verifying its involvement in a stabilizing hydrogen bond network. We also demonstrate that varv F binds to dodecylphosphocholine micelles, defining the binding orientation and showing that its structure remains unchanged upon binding, further demonstrating that the cyclotide fold is rigid. This study provides a biological insight into the mechanism by which cyclotides maintain their native activity in the unfavorable environment of predator insect guts. It also provides a structural basis for explaining how a cluster of residues important for bioactivity may be involved in self-association interactions in membranes. As well as being important for their bioactivity, the structural rigidity of cyclotides makes them very suitable as a stable template for peptide-based drug design.

PubMed: 19211551
DOI: 10.1074/jbc.M900021200

実験手法

X-RAY DIFFRACTION (1.8 Å)