3E47

Crystal Structure of the Yeast 20S Proteasome in Complex with Homobelactosin C

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3E47 の概要

• エントリーDOI: 10.2210/pdb3e47/pdb
• 関連するPDBエントリー: 1ryp
• 分子名称: Proteasome component Y7, Proteasome component C11, Proteasome component PRE2, ... (16 entities in total)
• 機能のキーワード: proteasome, ubiquitin, protein degradation, inhibitor, immunology, cytoplasm, hydrolase, nucleus, phosphoprotein, protease, threonine protease, ubl conjugation, zymogen
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: P23639 P22141 P30656 P23724 P30657 P38624 P23638 P40303 P32379 P40302 P21242 P21243 P25043 P25451
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 705401.64

構造登録者

Groll, M.,Larionov, O.V.,de Meijere, A. (登録日: 2008-08-10, 公開日: 2008-09-02, 最終更新日: 2024-10-09)

主引用文献

Groll, M.,Larionov, O.V.,Huber, R.,de Meijere, A.
Inhibitor-binding mode of homobelactosin C to proteasomes: new insights into class I MHC ligand generation
Proc.Natl.Acad.Sci.Usa, 103:4576-4579, 2006

PubMed Abstract: Most class I MHC ligands are generated from the vast majority of cellular proteins by proteolysis within the ubiquitin-proteasome pathway and are presented on the cell surface by MHC class I molecules. Here, we present the crystallographic analysis of yeast 20S proteasome in complex with the inhibitor homobelactosin C. The structure reveals a unique inhibitor-binding mode and provides information about the composition of proteasomal primed substrate-binding sites. IFN-gamma inducible substitution of proteasomal constitutive subunits by immunosubunits modulates characteristics of generated peptides, thus producing fragments with higher preference for binding to MHC class I molecules. The structural data for the proteasome:homobelactosin C complex provide an explanation for involvement of immunosubunits in antigen generation and open perspectives for rational design of ligands, inhibiting exclusively constitutive proteasomes or immunoproteasomes.

PubMed: 16537370
DOI: 10.1073/pnas.0600647103

実験手法

X-RAY DIFFRACTION (3 Å)