3E3S

Structure of thaumatin with the magic triangle I3C

Summary for 3E3S

• Entry DOI: 10.2210/pdb3e3s/pdb
• Related: 3E3D 3E3T
• Descriptor: Thaumatin I, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, L(+)-TARTARIC ACID, ... (5 entities in total)
• Functional Keywords: phasing tool, 5-amino-2, 4, 6-triiodoisophthalic acid, magic triangle, i3c, plant protein
• Biological source: Thaumatococcus daniellii (katemfe,miracle fruit)
• Total number of polymer chains: 1
• Total formula weight: 25328.70

Authors

Beck, T.,Gruene, T.,Sheldrick, G.M. (deposition date: 2008-08-08, release date: 2008-10-28, Last modification date: 2024-10-30)

Primary citation

Beck, T.,Krasauskas, A.,Gruene, T.,Sheldrick, G.M.
A magic triangle for experimental phasing of macromolecules
Acta Crystallogr.,Sect.D, 64:1179-1182, 2008

PubMed Abstract: Obtaining phase information for the solution of macromolecular structures is still one of the bottlenecks in X-ray crystallography. 5-Amino-2,4,6-triiodoisophthalic acid (I3C), in which three covalently bound iodines form an equilateral triangle, was incorporated into proteins in order to obtain phases by single-wavelength anomalous dispersion (SAD). An improved binding capability compared with simple heavy-metal ions, ready availability, improved recognition of potential heavy-atom sites and low toxicity make I3C particularly suitable for experimental phasing.

PubMed: 19020357
DOI: 10.1107/S0907444908030266

Experimental method

X-RAY DIFFRACTION (1.73 Å)