3E3S

Structure of thaumatin with the magic triangle I3C

3E3S の概要

• エントリーDOI: 10.2210/pdb3e3s/pdb
• 関連するPDBエントリー: 3E3D 3E3T
• 分子名称: Thaumatin I, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, L(+)-TARTARIC ACID, ... (5 entities in total)
• 機能のキーワード: phasing tool, 5-amino-2, 4, 6-triiodoisophthalic acid, magic triangle, i3c, plant protein
• 由来する生物種: Thaumatococcus daniellii (katemfe,miracle fruit)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25328.70

構造登録者

Beck, T.,Gruene, T.,Sheldrick, G.M. (登録日: 2008-08-08, 公開日: 2008-10-28, 最終更新日: 2024-10-30)

主引用文献

Beck, T.,Krasauskas, A.,Gruene, T.,Sheldrick, G.M.
A magic triangle for experimental phasing of macromolecules
Acta Crystallogr.,Sect.D, 64:1179-1182, 2008

PubMed Abstract: Obtaining phase information for the solution of macromolecular structures is still one of the bottlenecks in X-ray crystallography. 5-Amino-2,4,6-triiodoisophthalic acid (I3C), in which three covalently bound iodines form an equilateral triangle, was incorporated into proteins in order to obtain phases by single-wavelength anomalous dispersion (SAD). An improved binding capability compared with simple heavy-metal ions, ready availability, improved recognition of potential heavy-atom sites and low toxicity make I3C particularly suitable for experimental phasing.

PubMed: 19020357
DOI: 10.1107/S0907444908030266

実験手法

X-RAY DIFFRACTION (1.73 Å)