3E3R

Crystal structure and biochemical characterization of recombinant human calcyphosine delineates a novel EF-hand-containing protein family

3E3R の概要

• エントリーDOI: 10.2210/pdb3e3r/pdb
• 分子名称: Calcyphosin, CALCIUM ION (3 entities in total)
• 機能のキーワード: human calcyphosine, ef-hand, phosphoprotein, calcium binding protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm: Q13938
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45324.92

構造登録者

Dong, H. (登録日: 2008-08-07, 公開日: 2008-08-26, 最終更新日: 2024-03-20)

主引用文献

Dong, H.,Li, X.,Lou, Z.,Xu, X.,Su, D.,Zhou, X.,Zhou, W.,Bartlam, M.,Rao, Z.
Crystal-structure and biochemical characterization of recombinant human calcyphosine delineates a novel EF-hand-containing protein family
J.Mol.Biol., 383:455-464, 2008

PubMed Abstract: Calcyphosine is an EF-hand protein involved in both Ca(2+)-phosphatidylinositol and cyclic AMP signal cascades, as well as in other cellular functions. The crystal structure of Ca(2+)-loaded calcyphosine was determined up to 2.65 A resolution and reveals a protein containing two pairs of Ca(2+)-binding EF-hand motifs. Calcyphosine shares a highly similar overall topology with calmodulin. However, there are striking differences between EF-hand 4, both N-terminal and C-terminal regions, and interdomain linkers. The C-terminal domain of calcyphosine possesses a large hydrophobic pocket in the presence of calcium ions that might be implicated in ligand binding, while its N-terminal hydrophobic pocket is almost shielded by an additional terminal helix. Calcyphosine is largely monomeric, regardless of the presence of Ca(2+). Differences in structure, oligomeric state in the presence and in the absence of Ca(2+), a highly conserved sequence with low similarity to other proteins, and phylogeny define a new EF-hand-containing family of calcyphosine proteins that extends from arthropods to humans.

PubMed: 18775726
DOI: 10.1016/j.jmb.2008.08.048

実験手法

X-RAY DIFFRACTION (2.65 Å)