3DW6

Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-SECH3 modified

Summary for 3DW6

• Entry DOI: 10.2210/pdb3dw6/pdb
• Related: 1q9a 3DVZ 3DW4 3DW5 3DW7 483D
• Descriptor: Sarcin/Ricin Domain from E. Coli 23 S rRNA, GLYCEROL (3 entities in total)
• Functional Keywords: rna, sarcin ricin loop
• Total number of polymer chains: 1
• Total formula weight: 8913.33

Authors

Olieric, V.,Rieder, U.,Lang, K.,Serganov, A.,Schulze-Briese, C.,Micura, R.,Dumas, P.,Ennifar, E. (deposition date: 2008-07-21, release date: 2009-03-24, Last modification date: 2024-02-21)

Primary citation

Olieric, V.,Rieder, U.,Lang, K.,Serganov, A.,Schulze-Briese, C.,Micura, R.,Dumas, P.,Ennifar, E.
A fast selenium derivatization strategy for crystallization and phasing of RNA structures.
Rna, 15:707-715, 2009

PubMed Abstract: Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.

PubMed: 19228585
DOI: 10.1261/rna.1499309

Experimental method

X-RAY DIFFRACTION (1 Å)