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3DVK

Crystal Structure of Ca2+/CaM-CaV2.3 IQ domain complex

Summary for 3DVK
Entry DOI10.2210/pdb3dvk/pdb
Related3DVE 3DVJ 3DVM
DescriptorCalmodulin, Voltage-dependent R-type calcium channel subunit alpha-1E, CALCIUM ION, ... (4 entities in total)
Functional Keywordscalmodulin, calcium channel, iq domain, inactivation, facilitation, calcium-dependent, voltage-gated, membrane protein
Biological sourceHomo sapiens (human)
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Cellular locationMembrane; Multi-pass membrane protein: Q07652
Total number of polymer chains2
Total formula weight19610.03
Authors
Kim, E.Y.,Rumpf, C.H.,Fujiwara, Y.,Cooley, E.S.,Van Petegem, F.,Minor, D.L. (deposition date: 2008-07-18, release date: 2008-11-04, Last modification date: 2024-02-21)
Primary citationKim, E.Y.,Rumpf, C.H.,Fujiwara, Y.,Cooley, E.S.,Van Petegem, F.,Minor, D.L.
Structures of Ca(V)2 Ca(2+)/CaM-IQ Domain Complexes Reveal Binding Modes that Underlie Calcium-Dependent Inactivation and Facilitation.
Structure, 16:1455-1467, 2008
Cited by
PubMed Abstract: Calcium influx drives two opposing voltage-activated calcium channel (Ca(V)) self-modulatory processes: calcium-dependent inactivation (CDI) and calcium-dependent facilitation (CDF). Specific Ca(2+)/calmodulin (Ca(2+)/CaM) lobes produce CDI and CDF through interactions with the Ca(V)alpha(1) subunit IQ domain. Curiously, Ca(2+)/CaM lobe modulation polarity appears inverted between Ca(V)1s and Ca(V)2s. Here, we present crystal structures of Ca(V)2.1, Ca(V)2.2, and Ca(V)2.3 Ca(2+)/CaM-IQ domain complexes. All display binding orientations opposite to Ca(V)1.2 with a physical reversal of the CaM lobe positions relative to the IQ alpha-helix. Titration calorimetry reveals lobe competition for a high-affinity site common to Ca(V)1 and Ca(V)2 IQ domains that is occupied by the CDI lobe in the structures. Electrophysiological experiments demonstrate that the N-terminal Ca(V)2 Ca(2+)/C-lobe anchors affect CDF. Together, the data unveil the remarkable structural plasticity at the heart of Ca(V) feedback modulation and indicate that Ca(V)1 and Ca(V)2 IQ domains bear a dedicated CDF site that exchanges Ca(2+)/CaM lobe occupants.
PubMed: 18940602
DOI: 10.1016/j.str.2008.07.010
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.3 Å)
Structure validation

226707

數據於2024-10-30公開中

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