3DTI

Crystal structure of the IRRE protein, a central regulator of DNA damage repair in deinococcaceae

3DTI の概要

• エントリーDOI: 10.2210/pdb3dti/pdb
• 関連するPDBエントリー: 3DTE 3DTK
• 分子名称: IRRE protein, ZINC ION (2 entities in total)
• 機能のキーワード: irre, deinococcus, radiotolerance, gene regulation, metallopeptidase
• 由来する生物種: deinococcus deserti
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32402.56

構造登録者

Vujicic-Zagar, A.,Dulermo, R.,Le Gorrec, M.,Vannier, F.,Servant, P.,Sommer, S.,De Groot, A.,Serre, L. (登録日: 2008-07-15, 公開日: 2009-02-10, 最終更新日: 2023-11-01)

主引用文献

Vujicic-Zagar, A.,Dulermo, R.,Le Gorrec, M.,Vannier, F.,Servant, P.,Sommer, S.,de Groot, A.,Serre, L.
Crystal structure of the IrrE protein, a central regulator of DNA damage repair in deinococcaceae
J.Mol.Biol., 386:704-716, 2009

PubMed Abstract: Deinococcaceae are famous for their extreme radioresistance. Transcriptome analysis in Deinococcus radiodurans revealed a group of genes up-regulated in response to desiccation and ionizing radiation. IrrE, a novel protein initially found in D. radiodurans, was shown to be a positive regulator of some of these genes. Deinococcus deserti irrE is able to restore radioresistance in a D. radiodurans DeltairrE mutant. The D. deserti IrrE crystal structure reveals a unique combination of three domains: one zinc peptidase-like domain, one helix-turn-helix motif and one GAF-like domain. Mutant analysis indicates that the first and third domains are critical regions for radiotolerance. In particular, mutants affected in the putative zinc-binding site are as sensitive to gamma and UV irradiation as the DeltairrE bacteria, and radioresistance is strongly decreased with the H217L mutation present in the C-terminal domain. In addition, modeling of IrrE-DNA interaction suggests that the observed IrrE structure may not bind double-stranded DNA through its central helix-turn-helix motif and that IrrE is not a classic transcriptional factor that activates gene expression by its direct binding to DNA. We propose that the putative protease activity of IrrE could be a key element of transcription enhancement and that a more classic transcription factor, possibly an IrrE substrate, would link IrrE to transcription of genes specifically involved in radioresistance.

PubMed: 19150362
DOI: 10.1016/j.jmb.2008.12.062

実験手法

X-RAY DIFFRACTION (3.5 Å)