3DRZ

X-ray crystal structure of the N-terminal BTB domain of human KCTD5 protein

3DRZ の概要

• エントリーDOI: 10.2210/pdb3drz/pdb
• 関連するPDBエントリー: 3DRX 3DRY
• 分子名称: BTB/POZ domain-containing protein KCTD5 (2 entities in total)
• 機能のキーワード: kctd5, btb/poz, potassium channel domain t1, pentamer, unknown function
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytosol: Q9NXV2
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 61604.84

構造登録者

Tereshko, V.,Dementieva, I.,Goldstein, S.A.N. (登録日: 2008-07-11, 公開日: 2009-02-17, 最終更新日: 2024-11-13)

主引用文献

Dementieva, I.S.,Tereshko, V.,McCrossan, Z.A.,Solomaha, E.,Araki, D.,Xu, C.,Grigorieff, N.,Goldstein, S.A.
Pentameric assembly of potassium channel tetramerization domain-containing protein 5.
J.Mol.Biol., 387:175-191, 2009

PubMed Abstract: We report the X-ray crystal structure of human potassium channel tetramerization domain-containing protein 5 (KCTD5), the first member of the family to be so characterized. Four findings were unexpected. First, the structure reveals assemblies of five subunits while tetramers were anticipated; pentameric stoichiometry is observed also in solution by scanning transmission electron microscopy mass analysis and analytical ultracentrifugation. Second, the same BTB (bric-a-brac, tramtrack, broad complex) domain surface mediates the assembly of five KCTD5 and four voltage-gated K(+) (Kv) channel subunits; four amino acid differences appear crucial. Third, KCTD5 complexes have well-defined N- and C-terminal modules separated by a flexible linker that swivels by approximately 30 degrees; the C-module shows a new fold and is required to bind Golgi reassembly stacking protein 55 with approximately 1 microM affinity, as judged by surface plasmon resonance and ultracentrifugation. Fourth, despite the homology reflected in its name, KCTD5 does not impact the operation of Kv4.2, Kv3.4, Kv2.1, or Kv1.2 channels.

PubMed: 19361449
DOI: 10.1016/j.jmb.2009.01.030

実験手法

X-RAY DIFFRACTION (1.9 Å)