3DR2

Structural and Functional Analyses of XC5397 from Xanthomonas campestris: A Gluconolactonase Important in Glucose Secondary Metabolic Pathways

3DR2 の概要

• エントリーDOI: 10.2210/pdb3dr2/pdb
• 分子名称: Exported gluconolactonase, CALCIUM ION (3 entities in total)
• 機能のキーワード: xanthomonas campestris, gluconolactonase crystal structure, smp-30, six-bladed-propeller dimer, vitamin c, hydrolase
• 由来する生物種: Xanthomonas campestris pv. campestris
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66784.71

構造登録者

Chen, C.-N.,Chin, K.-H.,Wang, A.H.-J.,Chou, S.H. (登録日: 2008-07-10, 公開日: 2008-10-07, 最終更新日: 2024-10-30)

主引用文献

Chen, C.-N.,Chin, K.-H.,Wang, A.H.-J.,Chou, S.-H.
The First Crystal Structure of Gluconolactonase Important in the Glucose Secondary Metabolic Pathways
J.Mol.Biol., 384:604-614, 2008

PubMed Abstract: The first gluconolactonase crystal structure from bacteria has been determined to a resolution of 1.61 A using X-ray crystallography. It belongs to the senescence marker protein 30/gluconolaconase superfamily but exhibits substrate specificity mainly toward D-glucono-delta-lactone. It forms a novel disulfide-bonded clamshell dimer comprising two doughnut-shaped six-bladed beta-propeller domains, yet with an exceptionally long N-terminal subdomain forming an extra helix and four additional beta-strands to enclose half of the outermost beta-strands of each propeller. Extensive interactions, including H-bonds, salt bridges, disulfide bonds, and coordination bonds, along with numerous bridging water molecules, are present in the interface to institute the "top-to-top" clamshell-type dimer. Three calcium ions per subunit were observed. Two are present in the central water-filled channel, with the top one coordinated to four highly conserved amino acids and is possibly involved in substrate hydrolysis, while the bottom one is coordinated to the backbone oxygen atoms, which is possibly for stabilizing the propeller domain. One calcium ion is situated in the interface also to stabilize the dimer form. Since gluconolactonase is essential in the glucose secondary metabolic pathways leading to the synthesis of pentose, vitamin C, or "antiaging" factors, determination of its tertiary structure should help understand these important biochemical processes.

PubMed: 18848569
DOI: 10.1016/j.jmb.2008.09.055

実験手法

X-RAY DIFFRACTION (1.67 Å)