3DKN

Sec61 in the Canine ribosome-channel complex from the endoplasmic reticulum

3DKN の概要

• エントリーDOI: 10.2210/pdb3dkn/pdb
• EMDBエントリー: 1528
• 分子名称: RNA (5'-R(P*CP*GP*UP*GP*CP*CP*AP*AP*GP*CP*UP*GP*CP*GP*AP*UP*AP*AP*GP*C)-3'), RNA (5'-R(P*AP*GP*CP*CP*GP*CP*AP*CP*GP*GP*AP*GP*GP*CP*GP*AP*A)-3'), RNA (32-MER), ... (6 entities in total)
• 機能のキーワード: ribosome-channel complex, co-translational translocation, endoplasmic reticulum, protein transport-rna complex, protein transport/rna
• 由来する生物種: Canis lupus familiaris; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 80071.60

構造登録者

Menetret, J.-F.,Akey, C. (登録日: 2008-06-25, 公開日: 2008-08-19, 最終更新日: 2024-02-21)

主引用文献

Menetret, J.F.,Hegde, R.S.,Aguiar, M.,Gygi, S.P.,Park, E.,Rapoport, T.A.,Akey, C.W.
Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.
Structure, 16:1126-1137, 2008

PubMed Abstract: During cotranslational protein translocation, the ribosome associates with a membrane channel, formed by the Sec61 complex, and recruits the translocon-associated protein complex (TRAP). Here we report the structure of a ribosome-channel complex from mammalian endoplasmic reticulum in which the channel has been visualized at 11 A resolution. In this complex, single copies of Sec61 and TRAP associate with a nontranslating ribosome and this stoichiometry was verified by quantitative mass spectrometry. A bilayer-like density surrounds the channel and can be attributed to lipid and detergent. The crystal structure of an archaeal homolog of the Sec61 complex was then docked into the map. In this model, two cytoplasmic loops of Sec61 may interact with RNA helices H6, H7, and H50, while the central pore is located below the ribosome tunnel exit. Hence, this copy of Sec61 is positioned to capture and translocate the nascent chain. Finally, we show that mammalian and bacterial ribosome-channel complexes have similar architectures.

PubMed: 18611385
DOI: 10.1016/j.str.2008.05.003

実験手法

ELECTRON MICROSCOPY (8.7 Å)