3DF6

The thermo- and acido-stable ORF-99 from the archaeal virus AFV1

3DF6 の概要

• エントリーDOI: 10.2210/pdb3df6/pdb
• 分子名称: ORF99, CALCIUM ION (3 entities in total)
• 機能のキーワード: archaeal virus, extremophiles, protein fold, lipothrixviridae, viral protein, unknown function
• 由来する生物種: Acidianus Filamentous Virus 1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47287.86

構造登録者

Goulet, A.,Spinelli, S.,Prangishvili, D.,van Tilbeurgh, H.,Cambillau, C.,Campanacci, V. (登録日: 2008-06-11, 公開日: 2009-06-16, 最終更新日: 2024-10-30)

主引用文献

Goulet, A.,Spinelli, S.,Blangy, S.,van Tilbeurgh, H.,Leulliot, N.,Basta, T.,Prangishvili, D.,Cambillau, C.,Campanacci, V.
The thermo- and acido-stable ORF-99 from the archaeal virus AFV1
Protein Sci., 18:1316-1320, 2009

PubMed Abstract: Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 has a new fold, is hyperthermostable (up to 95 degrees C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95 degrees C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues.

PubMed: 19472363
DOI: 10.1002/pro.122

実験手法

X-RAY DIFFRACTION (2.05 Å)