3DEE

CRYSTAL STRUCTURE OF A PUTATIVE REGULATORY PROTEIN INVOLVED IN TRANSCRIPTION (NGO1945) FROM NEISSERIA GONORRHOEAE FA 1090 AT 2.25 A RESOLUTION

Summary for 3DEE

• Entry DOI: 10.2210/pdb3dee/pdb
• Descriptor: Putative regulatory protein, CHLORIDE ION, IMIDAZOLE, ... (4 entities in total)
• Functional Keywords: putative regulatory protein, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, transcription
• Biological source: Neisseria gonorrhoeae FA 1090
• Total number of polymer chains: 1
• Total formula weight: 29145.34

Authors

Joint Center for Structural Genomics (JCSG) (deposition date: 2008-06-09, release date: 2008-08-05, Last modification date: 2024-10-09)

Primary citation

Das, D.,Grishin, N.V.,Kumar, A.,Carlton, D.,Bakolitsa, C.,Miller, M.D.,Abdubek, P.,Astakhova, T.,Axelrod, H.L.,Burra, P.,Chen, C.,Chiu, H.J.,Chiu, M.,Clayton, T.,Deller, M.C.,Duan, L.,Ellrott, K.,Ernst, D.,Farr, C.L.,Feuerhelm, J.,Grzechnik, A.,Grzechnik, S.K.,Grant, J.C.,Han, G.W.,Jaroszewski, L.,Jin, K.K.,Johnson, H.A.,Klock, H.E.,Knuth, M.W.,Kozbial, P.,Krishna, S.S.,Marciano, D.,McMullan, D.,Morse, A.T.,Nigoghossian, E.,Nopakun, A.,Okach, L.,Oommachen, S.,Paulsen, J.,Puckett, C.,Reyes, R.,Rife, C.L.,Sefcovic, N.,Tien, H.J.,Trame, C.B.,van den Bedem, H.,Weekes, D.,Wooten, T.,Xu, Q.,Hodgson, K.O.,Wooley, J.,Elsliger, M.A.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Wilson, I.A.
The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation.
Acta Crystallogr.,Sect.F, 66:1174-1181, 2010

PubMed Abstract: Proteins with the DUF2063 domain constitute a new Pfam family, PF09836. The crystal structure of a member of this family, NGO1945 from Neisseria gonorrhoeae, has been determined and reveals that the N-terminal DUF2063 domain is likely to be a DNA-binding domain. In conjunction with the rest of the protein, NGO1945 is likely to be involved in transcriptional regulation, which is consistent with genomic neighborhood analysis. Of the 216 currently known proteins that contain a DUF2063 domain, the most significant sequence homologs of NGO1945 (∼40-99% sequence identity) are from various Neisseria and Haemophilus species. As these are important human pathogens, NGO1945 represents an interesting candidate for further exploration via biochemical studies and possible therapeutic intervention.

PubMed: 20944208
DOI: 10.1107/S1744309109022672

Experimental method

X-RAY DIFFRACTION (2.1 Å)