3DE1

Proteinase K by LB nanotemplate method after the third step of high X-Ray dose on ESRF ID23-1 beamline

3DE1 の概要

• エントリーDOI: 10.2210/pdb3de1/pdb
• 関連するPDBエントリー: 3D9Q 3DDZ 3DE0 3DE2 3DE3 3DE4 3DE5 3DE6 3DE7
• 分子名称: Proteinase K, CALCIUM ION (3 entities in total)
• 機能のキーワード: alpha beta protein, hydrolase, calcium, metal-binding, protease, serine protease, zymogen
• 由来する生物種: Engyodontium album (Engyodontium album)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28970.86

構造登録者

Pechkova, E.,Tripathi, S.K.,Nicolini, C. (登録日: 2008-06-07, 公開日: 2009-06-09, 最終更新日: 2024-10-09)

主引用文献

Pechkova, E.,Tripathi, S.,Ravelli, R.B.,McSweeney, S.,Nicolini, C.
Radiation stability of proteinase K crystals grown by LB nanotemplate method
J.Struct.Biol., 168:409-418, 2009

PubMed Abstract: A detailed analysis of structural and intensity changes induced by X-ray radiation is presented for two types of proteinase K crystals: crystal grown by classical hanging drop method and those grown by Langmuir-Blodgett (LB) nanotemplate. The comparison of various parameters (e.g. intensity per sigma ratio, unit-cell volume, number of unique reflections, B-factors) and electron density maps as a function of radiation dose, demonstrates that crystals, grown by the LB nanotemplate method, appear to be more resistant against radiation damage than crystals grown by the classical hanging drop method.

PubMed: 19686853
DOI: 10.1016/j.jsb.2009.08.005

実験手法

X-RAY DIFFRACTION (2 Å)