3DDR

Structure of the Serratia marcescens hemophore receptor HasR-Ile671Gly mutant in complex with its hemophore HasA and heme

3DDR の概要

• エントリーDOI: 10.2210/pdb3ddr/pdb
• 関連するPDBエントリー: 3CSL 3CSN
• 分子名称: HasR protein, Hemophore HasA, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: outer membrane protein, beta-barrel, hemophore receptor, membrane, outer membrane, tonb box, heme, iron, metal-binding, secreted, membrane protein-heme binding protein complex, membrane protein/heme binding protein
• 由来する生物種: Serratia marcescens; 詳細
• 細胞内の位置: Cell outer membrane : Q79AD2; Secreted : Q54450
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 234675.21

構造登録者

Krieg, S.,Diederichs, K. (登録日: 2008-06-06, 公開日: 2009-01-20, 最終更新日: 2024-11-13)

主引用文献

Krieg, S.,Huche, F.,Diederichs, K.,Izadi-Pruneyre, N.,Lecroisey, A.,Wandersman, C.,Delepelaire, P.,Welte, W.
Heme uptake across the outer membrane as revealed by crystal structures of the receptor-hemophore complex.
Proc.Natl.Acad.Sci.USA, 106:1045-1050, 2009

PubMed Abstract: Gram-negative bacteria use specific heme uptake systems, relying on outer membrane receptors and excreted heme-binding proteins (hemophores) to scavenge and actively transport heme. To unravel the unknown molecular details involved, we present 3 structures of the Serratia marcescens receptor HasR in complex with its hemophore HasA. The transfer of heme over a distance of 9 A from its high-affinity site in HasA into a site of lower affinity in HasR is coupled with the exergonic complex formation of the 2 proteins. Upon docking to the receptor, 1 of the 2 axial heme coordinations of the hemophore is initially broken, but the position and orientation of the heme is preserved. Subsequently, steric displacement of heme by a receptor residue ruptures the other axial coordination, leading to heme transfer into the receptor.

PubMed: 19144921
DOI: 10.1073/pnas.0809406106

実験手法

X-RAY DIFFRACTION (2.8 Å)