3D38

Crystal structure of new trigonal form of photosynthetic reaction center from Blastochloris viridis. Crystals grown in microfluidics by detergent capture.

3D38 の概要

• エントリーDOI: 10.2210/pdb3d38/pdb
• 分子名称: Photosynthetic reaction center cytochrome c subunit, BACTERIOPHEOPHYTIN B, UBIQUINONE-1, ... (15 entities in total)
• 機能のキーワード: detergent extraction, reaction center, microfludics, plugs, structural genomics, psi-2, protein structure initiative, accelerated technologies center for gene to 3d structure, atcg3d, electron transport, heme, iron, lipoprotein, membrane, metal-binding, photosynthesis, transport, bacteriochlorophyll, chlorophyll, chromophore, formylation, transmembrane, magnesium
• 由来する生物種: Blastochloris viridis; 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor: P07173; Cellular chromatophore membrane; Single-pass membrane protein: P06008; Cellular chromatophore membrane; Multi-pass membrane protein (By similarity): P06009 P06010
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 144981.62

構造登録者

Li, L.,Nachtergaele, S.H.M.,Seddon, A.M.,Tereshko, V.,Ponomarenko, N.,Ismagilov, R.F.,Accelerated Technologies Center for Gene to 3D Structure (ATCG3D) (登録日: 2008-05-09, 公開日: 2008-07-08, 最終更新日: 2024-11-06)

主引用文献

Li, L.,Nachtergaele, S.,Seddon, A.M.,Tereshko, V.,Ponomarenko, N.,Ismagilov, R.F.
Simple host-guest chemistry to modulate the process of concentration and crystallization of membrane proteins by detergent capture in a microfluidic device.
J.Am.Chem.Soc., 130:14324-14328, 2008

PubMed Abstract: This paper utilizes cyclodextrin-based host-guest chemistry in a microfluidic device to modulate the crystallization of membrane proteins and the process of concentration of membrane protein samples. Methyl-beta-cyclodextrin (MBCD) can efficiently capture a wide variety of detergents commonly used for the stabilization of membrane proteins by sequestering detergent monomers. Reaction Center (RC) from Blastochloris viridis was used here as a model system. In the process of concentrating membrane protein samples, MBCD was shown to break up free detergent micelles and prevent them from being concentrated. The addition of an optimal amount of MBCD to the RC sample captured loosely bound detergent from the protein-detergent complex and improved sample homogeneity, as characterized by dynamic light scattering. Using plug-based microfluidics, RC crystals were grown in the presence of MBCD, giving a different morphology and space group than crystals grown without MBCD. The crystal structure of RC crystallized in the presence of MBCD was consistent with the changes in packing and crystal contacts hypothesized for removal of loosely bound detergent. The incorporation of MBCD into a plug-based microfluidic crystallization method allows efficient use of limited membrane protein sample by reducing the amount of protein required and combining sparse matrix screening and optimization in one experiment. The use of MBCD for detergent capture can be expanded to develop cyclodextrin-derived molecules for fine-tuned detergent capture and thus modulate membrane protein crystallization in an even more controllable way.

PubMed: 18831551
DOI: 10.1021/ja805361j

実験手法

X-RAY DIFFRACTION (3.21 Å)