3CZV

Crystal structure of the human carbonic anhydrase XIII in complex with acetazolamide

3CZV の概要

• エントリーDOI: 10.2210/pdb3czv/pdb
• 関連するPDBエントリー: 1CA2
• 分子名称: Carbonic anhydrase 13, ZINC ION, 5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE, ... (5 entities in total)
• 機能のキーワード: carbonic anhydrase, protein-inhibitor complex, lyase, metal-binding
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59919.91

構造登録者

Di Fiore, A.,De Simone, G. (登録日: 2008-04-30, 公開日: 2008-07-29, 最終更新日: 2024-04-03)

主引用文献

Di Fiore, A.,Monti, S.M.,Hilvo, M.,Parkkila, S.,Romano, V.,Scaloni, A.,Pedone, C.,Scozzafava, A.,Supuran, C.T.,De Simone, G.
Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide.
Proteins, 74:164-175, 2008

PubMed Abstract: The cytosolic isoform XIII is a recently discovered member of the human carbonic anhydrase (hCA, EC 4.2.1.1) family. It is selectively expressed among other tissues in the reproductive organs, where it may control pH and ion balance regulation, ensuring thus proper fertilization conditions. The authors report here the X-ray crystallographic structure of this isozyme in the unbound state and in complex with a classical sulfonamide inhibitor, namely acetazolamide. A detailed comparison of the obtained structural data with those already reported for other CA isozymes provides novel insights into the catalytic properties of the members of this protein family. On the basis of the inhibitory properties of acetazolamide against various cytosolic/transmembrane isoforms and the structural differences detected within the active site of the various CA isoforms, further prospects for the design of isozyme-specific CA inhibitors are here proposed.

PubMed: 18618712
DOI: 10.1002/prot.22144

実験手法

X-RAY DIFFRACTION (2 Å)