3CYU

Human Carbonic Anhydrase II complexed with Cryptophane biosensor and xenon

3CYU の概要

• エントリーDOI: 10.2210/pdb3cyu/pdb
• 分子名称: Carbonic anhydrase 2, ZINC ION, MoMo-2-[4-(2-(4-(methoxy)-1H-1,2,3-triazol-1-yl)ethyl)benzenesulfonamide]-7,12-bis-[3-(4-(methoxy)-1H-1,2,3-triazol-1-y l)propanoic acid]-cryptophane-A, ... (6 entities in total)
• 機能のキーワード: zinc metalloenzyme, sulfonamide, cryptophane, xenon, biosensor, disease mutation, lyase, metal-binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P00918
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32464.06

構造登録者

Aaron, J.A.,Jude, K.M.,Di Costanzo, L.,Christianson, D.W. (登録日: 2008-04-26, 公開日: 2008-05-27, 最終更新日: 2023-08-30)

主引用文献

Aaron, J.A.,Chambers, J.M.,Jude, K.M.,Di Costanzo, L.,Dmochowski, I.J.,Christianson, D.W.
Structure of a 129Xe-cryptophane biosensor complexed with human carbonic anhydrase II.
J.Am.Chem.Soc., 130:6942-6943, 2008

PubMed Abstract: Cryptophanes represent an exciting class of xenon-encapsulating molecules that can be exploited as probes for nuclear magnetic resonance imaging. The 1.70 A resolution crystal structure of a cryptophane-derivatized benezenesulfonamide complexed with human carbonic anhydrase II shows how an encapsulated xenon atom can be directed to a specific biological target. The crystal structure confirms binding measurements indicating that the cryptophane cage does not strongly interact with the surface of the protein, which may enhance the sensitivity of 129Xe NMR spectroscopic measurements in solution.

PubMed: 18461940
DOI: 10.1021/ja802214x

実験手法

X-RAY DIFFRACTION (1.7 Å)