3CRO

THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION

Summary for 3CRO

• Entry DOI: 10.2210/pdb3cro/pdb
• Descriptor: DNA (5'-D(*AP*AP*GP*TP*AP*CP*AP*AP*AP*CP*TP*TP*TP*CP*TP*TP*G P*TP*AP*T)-3'), DNA (5'-D(*TP*AP*TP*AP*CP*AP*AP*GP*AP*AP*AP*GP*TP*TP*TP*GP*T P*AP*CP*T)-3'), PROTEIN (434 CRO), ... (4 entities in total)
• Functional Keywords: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• Biological source: Phage 434
• Total number of polymer chains: 4
• Total formula weight: 28416.25

Authors

Mondragon, A.,Harrison, S.C. (deposition date: 1990-07-06, release date: 1991-10-15, Last modification date: 2024-02-21)

Primary citation

Mondragon, A.,Harrison, S.C.
The phage 434 Cro/OR1 complex at 2.5 A resolution.
J.Mol.Biol., 219:321-334, 1991

PubMed Abstract: The crystal structure of phage 434 Cro protein in complex with a 20 base-pair DNA fragment has been determined to 2.5 A resolution. The DNA fragment contains the sequence of the OR1 operator site. The structure shows a bent conformation for the DNA, straighter at the center and more bent at the ends. The central base-pairs adopt conformations with significant deviations from coplanarity. The two molecules interact extensively along their common interface, both through hydrogen bonds and van der Waals interactions. The significance of these interactions for operator binding and recognition is discussed.

PubMed: 2038059
DOI: 10.1016/0022-2836(91)90568-Q

Experimental method

X-RAY DIFFRACTION (2.5 Å)