3CQG

Nucleoporin Nup107/Nup133 interaction complex, delta finger mutant

3CQG の概要

• エントリーDOI: 10.2210/pdb3cqg/pdb
• 関連するPDBエントリー: 3CQC
• 分子名称: Nuclear pore complex protein Nup107, Nuclear pore complex protein Nup133 (3 entities in total)
• 機能のキーワード: nucleoporins, nuclear pore complex, mrna transport, nucleus, phosphoprotein, protein transport, translocation
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus membrane : P57740; Nucleus, nuclear pore complex : Q8WUM0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54374.09

構造登録者

Jeudy, S.,Boehmer, T.,Berke, I.,Schwartz, T.U. (登録日: 2008-04-02, 公開日: 2008-07-01, 最終更新日: 2024-02-21)

主引用文献

Boehmer, T.,Jeudy, S.,Berke, I.C.,Schwartz, T.U.
Structural and functional studies of Nup107/Nup133 interaction and its implications for the architecture of the nuclear pore complex.
Mol.Cell, 30:721-731, 2008

PubMed Abstract: Nuclear pore complexes (NPCs) are 40-60 MDa protein assemblies embedded in the nuclear envelope of eukaryotic cells. NPCs exclusively mediate all transport between cytoplasm and nucleus. The nucleoporins that build the NPC are arranged in a stable core of module-like subcomplexes with eight-fold rotational symmetry. To gain insight into the intricate assembly of the NPC, we have solved the crystal structure of a protein complex between two nucleoporins, human Nup107 and Nup133. Both proteins form elongated structures that interact tightly via a compact interface in tail-to-tail fashion. Additional experiments using structure-guided mutants show that Nup107 is the critical anchor for Nup133 to the NPC, positioning Nup133 at the periphery of the NPC. The significant topological differences between Nup107 and Nup133 suggest that *-helical nucleoporin domains of the NPC scaffold fall in different classes and fulfill largely nonredundant functions.

PubMed: 18570875
DOI: 10.1016/j.molcel.2008.04.022

実験手法

X-RAY DIFFRACTION (3 Å)