3CML

Crystal Structure of the DBL3x domain of the Plasmodium falcipurum VAR2CSA protein

3CML の概要

• エントリーDOI: 10.2210/pdb3cml/pdb
• 分子名称: Erythrocyte membrane protein 1 (2 entities in total)
• 機能のキーワード: dbl3x, var2csa, chondroitin-sulfate binding domain, membrane protein
• 由来する生物種: Plasmodium falciparum (malaria parasite)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41460.73

構造登録者

Singh, K.,Gittis, A.G.,Nguyen, P.,Gowda, D.C.,Miller, L.H.,Garboczi, D.N. (登録日: 2008-03-23, 公開日: 2008-09-02, 最終更新日: 2024-10-30)

主引用文献

Singh, K.,Gittis, A.G.,Nguyen, P.,Gowda, D.C.,Miller, L.H.,Garboczi, D.N.
Structure of the DBL3x domain of pregnancy-associated malaria protein VAR2CSA complexed with chondroitin sulfate A.
Nat.Struct.Mol.Biol., 15:932-938, 2008

PubMed Abstract: Plasmodium falciparum-infected erythrocytes bind to chondroitin sulfate A (CSA) in the placenta via the VAR2CSA protein, a member of the P. falciparum erythrocyte membrane protein-1 family, leading to life-threatening malaria in pregnant women with severe effects on their fetuses and newborns. Here we describe the structure of the CSA binding DBL3x domain, a Duffy binding-like (DBL) domain of VAR2CSA. By forming a complex of DBL3x with CSA oligosaccharides and determining its structure, we have identified the CSA binding site to be a cluster of conserved positively charged residues on subdomain 2 and subdomain 3. Mutation or chemical modification of lysine residues at the site markedly diminished CSA binding to DBL3x. The location of the CSA binding site is an important step forward in the molecular understanding of pregnancy-associated malaria and offers a new target for vaccine development.

PubMed: 19172746
DOI: 10.1038/nsmb1479

実験手法

X-RAY DIFFRACTION (1.9 Å)