3CK4

A heterospecific leucine zipper tetramer

3CK4 の概要

• エントリーDOI: 10.2210/pdb3ck4/pdb
• 関連するPDBエントリー: 2B22 2IPZ 2ZTA
• 分子名称: GCN4 leucine zipper, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: coiled coils, anti-parallel tetramer, protein complex, heterospecific interaction, protein binding, activator, amino-acid biosynthesis, dna-binding, nucleus, phosphoprotein, transcription, transcription regulation
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: P03069 P03069
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 47938.00

構造登録者

Liu, J. (登録日: 2008-03-14, 公開日: 2008-10-07, 最終更新日: 2023-08-30)

主引用文献

Deng, Y.,Liu, J.,Zheng, Q.,Li, Q.,Kallenbach, N.R.,Lu, M.
A heterospecific leucine zipper tetramer.
Chem.Biol., 15:908-919, 2008

PubMed Abstract: Protein-protein interactions play an essential role in the assembly of the macromolecular complexes that form functional networks and control cellular behavior. Elucidating principles of molecular recognition governing potentially complex interfaces is a challenging goal for structural and systems biology. Extensive studies of alpha-helical coiled coils have provided fundamental insight into the determinants of one seemingly tractable class of oligomeric protein interfaces. We report here that two different valine-containing mutants of the GCN4 leucine zipper that fold individually as four-stranded coiled coils associate preferentially in mixtures to form an antiparallel, heterotetrameric structure. X-ray crystallographic analysis reveals that the coinciding hydrophobic interfaces of the hetero- and homotetramers differ in detail, thereby controlling their partnering and structural specificity. Equilibrium disulfide exchange and thermal denaturation experiments show that the 50-fold preference for heterospecificity is determined by interfacial van der Waals interactions and hydrophobicity. Parallel studies of two alanine-containing variants confirm the above-mentioned interpretation of the basis and mechanism of this heterospecificity. Our results suggest that coiled-coil recognition is an inherently geometric process in which heterotypic interaction specificity derives from a complementarity of both shape and chemistry.

PubMed: 18804028
DOI: 10.1016/j.chembiol.2008.07.008

実験手法

X-RAY DIFFRACTION (1.7 Å)