3CGH

Crystal structure of a susd homolog (bt_3984) from bacteroides thetaiotaomicron vpi-5482 at 1.70 A resolution

Summary for 3CGH

• Entry DOI: 10.2210/pdb3cgh/pdb
• Descriptor: SusD homolog, ZINC ION, ACETATE ION, ... (4 entities in total)
• Functional Keywords: structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, metal binding protein
• Biological source: Bacteroides thetaiotaomicron VPI-5482
• Total number of polymer chains: 1
• Total formula weight: 57690.65

Authors

Joint Center for Structural Genomics (JCSG) (deposition date: 2008-03-05, release date: 2008-03-18, Last modification date: 2024-10-09)

Primary citation

Bakolitsa, C.,Xu, Q.,Rife, C.L.,Abdubek, P.,Astakhova, T.,Axelrod, H.L.,Carlton, D.,Chen, C.,Chiu, H.J.,Clayton, T.,Das, D.,Deller, M.C.,Duan, L.,Ellrott, K.,Farr, C.L.,Feuerhelm, J.,Grant, J.C.,Grzechnik, A.,Han, G.W.,Jaroszewski, L.,Jin, K.K.,Klock, H.E.,Knuth, M.W.,Kozbial, P.,Krishna, S.S.,Kumar, A.,Lam, W.W.,Marciano, D.,McMullan, D.,Miller, M.D.,Morse, A.T.,Nigoghossian, E.,Nopakun, A.,Okach, L.,Puckett, C.,Reyes, R.,Tien, H.J.,Trame, C.B.,van den Bedem, H.,Weekes, D.,Hodgson, K.O.,Wooley, J.,Elsliger, M.A.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Wilson, I.A.
Structure of BT_3984, a member of the SusD/RagB family of nutrient-binding molecules.
Acta Crystallogr.,Sect.F, 66:1274-1280, 2010

PubMed Abstract: The crystal structure of the Bacteroides thetaiotaomicron protein BT_3984 was determined to a resolution of 1.7 Å and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT_3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT_3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.

PubMed: 20944222
DOI: 10.1107/S1744309110032999

Experimental method

X-RAY DIFFRACTION (1.7 Å)