3CAP

Crystal Structure of Native Opsin: the G Protein-Coupled Receptor Rhodopsin in its Ligand-free State

3CAP の概要

• エントリーDOI: 10.2210/pdb3cap/pdb
• 分子名称: Rhodopsin, beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: g protein-coupled receptor, opsin, rhodopsin, membrane protein, retinal protein, photoreceptor, ligand-free state, chromophore, g-protein coupled receptor, glycoprotein, lipoprotein, palmitate, phosphoprotein, photoreceptor protein, sensory transduction, transducer, transmembrane, vision, signaling protein
• 由来する生物種: Bos taurus (bovine)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P02699
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82676.14

構造登録者

Park, J.H.,Scheerer, P.,Hofmann, K.P.,Choe, H.-W.,Ernst, O.P. (登録日: 2008-02-20, 公開日: 2008-06-24, 最終更新日: 2024-11-20)

主引用文献

Park, J.H.,Scheerer, P.,Hofmann, K.P.,Choe, H.-W.,Ernst, O.P.
Crystal structure of the ligand-free G-protein-coupled receptor opsin
Nature, 454:183-187, 2008

PubMed Abstract: In the G-protein-coupled receptor (GPCR) rhodopsin, the inactivating ligand 11-cis-retinal is bound in the seven-transmembrane helix (TM) bundle and is cis/trans isomerized by light to form active metarhodopsin II. With metarhodopsin II decay, all-trans-retinal is released, and opsin is reloaded with new 11-cis-retinal. Here we present the crystal structure of ligand-free native opsin from bovine retinal rod cells at 2.9 ångström (A) resolution. Compared to rhodopsin, opsin shows prominent structural changes in the conserved E(D)RY and NPxxY(x)(5,6)F regions and in TM5-TM7. At the cytoplasmic side, TM6 is tilted outwards by 6-7 A, whereas the helix structure of TM5 is more elongated and close to TM6. These structural changes, some of which were attributed to an active GPCR state, reorganize the empty retinal-binding pocket to disclose two openings that may serve the entry and exit of retinal. The opsin structure sheds new light on ligand binding to GPCRs and on GPCR activation.

PubMed: 18563085
DOI: 10.1038/nature07063

実験手法

X-RAY DIFFRACTION (2.9 Å)