3C9K

Model of Histone Octamer Tubular Crystals

3C9K の概要

• エントリーDOI: 10.2210/pdb3c9k/pdb
• 関連するPDBエントリー: 2HIO
• EMDBエントリー: 1469
• 分子名称: Histone H2A-IV, Histone H2B 7, Histone H3.2, ... (4 entities in total)
• 機能のキーワード: helix, tubular crystal, chromosomal protein, nucleosome core, nucleus, phosphoprotein, methylation, dna binding protein
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 108510.80

構造登録者

Frouws, T.D. (登録日: 2008-02-16, 公開日: 2009-01-06, 最終更新日: 2024-03-13)

主引用文献

Frouws, T.D.,Patterton, H.G.,Sewell, B.T.
Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber
Biophys.J., 96:3363-3371, 2009

PubMed Abstract: A major question in chromatin involves the exact organization of nucleosomes within the 30-nm chromatin fiber and its structural determinants of assembly. Here we investigate the structure of histone octamer helical tubes via the method of iterative helical real-space reconstruction. Accurate placement of the x-ray structure of the histone octamer within the reconstructed density yields a pseudoatomic model for the entire helix, and allows precise identification of molecular interactions between neighboring octamers. One such interaction that would not be obscured by DNA in the nucleosome consists of a twofold symmetric four-helix bundle formed between pairs of H2B-alpha3 and H2B-alphaC helices of neighboring octamers. We believe that this interface can act as an internucleosomal four-helix bundle within the context of the chromatin fiber. The potential relevance of this interface in the folding of the 30-nm chromatin fiber is discussed.

PubMed: 19383479
DOI: 10.1016/j.bpj.2008.10.075

実験手法

ELECTRON MICROSCOPY (20 Å)