3C8P

X-ray structure of Viscotoxin A1 from Viscum album L.

3C8P の概要

• エントリーDOI: 10.2210/pdb3c8p/pdb
• 分子名称: Viscotoxin A1 (2 entities in total)
• 機能のキーワード: helix turn helix, toxin
• 由来する生物種: Viscum album (European Mistletoe)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 9793.26

構造登録者

Pal, A.,Debreczeni, J.E.,Sevvana, M.,Gruene, T.,Kahle, B.,Zeeck, A.,Sheldrick, G.M. (登録日: 2008-02-13, 公開日: 2008-06-17, 最終更新日: 2024-10-30)

主引用文献

Pal, A.,Debreczeni, J.E.,Sevvana, M.,Gruene, T.,Kahle, B.,Zeeck, A.,Sheldrick, G.M.
Structures of viscotoxins A1 and B2 from European mistletoe solved using native data alone
Acta Crystallogr.,Sect.D, 64:985-992, 2008

PubMed Abstract: Crystals of the cytotoxic thionin proteins viscotoxins A1 and B2 extracted from mistletoe diffracted to high resolution (1.25 and 1.05 A, respectively) and are excellent candidates for testing crystallographic methods. Ab initio direct methods were only successful in solving the viscotoxin B2 structure, which with 861 unique non-H atoms is one of the largest unknown structures without an atom heavier than sulfur to be solved in this way, but sulfur-SAD phasing provided a convincing solution for viscotoxin A1. Both proteins form dimers in the crystal and viscotoxin B2 (net charge +4 per monomer), but not viscotoxin A1 (net charge +6), is coordinated by sulfate or phosphate anions. The viscotoxin A1 crystal has a higher solvent content than the viscotoxin B2 crystal (49% as opposed to 28%) with solvent channels along the crystallographic 4(3) axes.

PubMed: 18703848
DOI: 10.1107/S0907444908022646

実験手法

X-RAY DIFFRACTION (1.25 Å)