3C7H

Crystal structure of glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase from Bacillus subtilis in complex with AXOS-4-0.5.

3C7H の概要

• エントリーDOI: 10.2210/pdb3c7h/pdb
• 関連するPDBエントリー: 3c7e 3c7f 3c7g 3c7o
• 関連するBIRD辞書のPRD_ID: PRD_900117
• 分子名称: Endo-1,4-beta-xylanase, beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose, CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: 5-bladed beta-propeller fold, beta-sandwich, xylan degradation, hydrolase
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Secreted : Q45071
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52636.84

構造登録者

Vandermarliere, E.,Bourgois, T.M.,Winn, M.D.,Van Campenhout, S.,Volckaert, G.,Strelkov, S.V.,Delcour, J.A.,Rabijns, A.,Courtin, C.M. (登録日: 2008-02-07, 公開日: 2008-11-18, 最終更新日: 2024-02-21)

主引用文献

Vandermarliere, E.,Bourgois, T.M.,Winn, M.D.,van Campenhout, S.,Volckaert, G.,Delcour, J.A.,Strelkov, S.V.,Rabijns, A.,Courtin, C.M.
Structural analysis of a glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase in complex with xylotetraose reveals a different binding mechanism compared with other members of the same family.
Biochem.J., 418:39-47, 2009

PubMed Abstract: AXHs (arabinoxylan arabinofuranohydrolases) are alpha-L-arabinofuranosidases that specifically hydrolyse the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Bacillus subtilis was recently shown to produce an AXH that cleaves arabinose units from O-2- or O-3-mono-substituted xylose residues: BsAXH-m2,3 (B. subtilis AXH-m2,3). Crystallographic analysis reveals a two-domain structure for this enzyme: a catalytic domain displaying a five-bladed beta-propeller fold characteristic of GH (glycoside hydrolase) family 43 and a CBM (carbohydrate-binding module) with a beta-sandwich fold belonging to CBM family 6. Binding of substrate to BsAXH-m2,3 is largely based on hydrophobic stacking interactions, which probably allow the positional flexibility needed to hydrolyse both arabinose substituents at the O-2 or O-3 position of the xylose unit. Superposition of the BsAXH-m2,3 structure with known structures of the GH family 43 exo-acting enzymes, beta-xylosidase and alpha-L-arabinanase, each in complex with their substrate, reveals a different orientation of the sugar backbone.

PubMed: 18980579
DOI: 10.1042/BJ20081256

実験手法

X-RAY DIFFRACTION (2 Å)