3C6D

The pseudo-atomic structure of dengue immature virus

3C6D の概要

• エントリーDOI: 10.2210/pdb3c6d/pdb
• 関連するPDBエントリー: 3C5X
• EMDBエントリー: 5102
• 分子名称: Polyprotein, prM (2 entities in total)
• 機能のキーワード: icosahedral virion, helicase, hydrolase, nucleotide-binding, rna replication, transmembrane, atp-binding, capsid protein, cleavage on pair of basic residues, endoplasmic reticulum, envelope protein, glycoprotein, metal-binding, multifunctional enzyme, nucleotidyltransferase, nucleus, phosphoprotein, protease, ribonucleoprotein, rna-binding, rna-directed rna polymerase, secreted, serine protease, transcription, transcription regulation, transferase, viral nucleoprotein, icosahedral virus, virus
• 由来する生物種: Dengue virus 2 Thailand/16681/84; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 159497.82

構造登録者

Li, L. (登録日: 2008-02-04, 公開日: 2008-04-08, 最終更新日: 2024-02-21)

主引用文献

Li, L.,Lok, S.M.,Yu, I.M.,Zhang, Y.,Kuhn, R.J.,Chen, J.,Rossmann, M.G.
The flavivirus precursor membrane-envelope protein complex: structure and maturation
Science, 319:1830-1834, 2008

PubMed Abstract: Many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of the precursor membrane protein (prM), turning inert virus into infectious particles. We have determined the 2.2 angstrom resolution crystal structure of a recombinant protein in which the dengue virus prM is linked to the envelope glycoprotein E. The structure represents the prM-E heterodimer and fits well into the cryo-electron microscopy density of immature virus at neutral pH. The pr peptide beta-barrel structure covers the fusion loop in E, preventing fusion with host cell membranes. The structure provides a basis for identifying the stages of its pH-directed conformational metamorphosis during maturation, ending with release of pr when budding from the host.

PubMed: 18369147
DOI: 10.1126/science.1153263

実験手法

ELECTRON MICROSCOPY (12.5 Å)