3C63

Tetrameric Cytochrome cb562 (K34/H59/D62/H63/H73/A74/H77) Assembly Stabilized by Interprotein Zinc Coordination

3C63 の概要

• エントリーDOI: 10.2210/pdb3c63/pdb
• 関連するPDBエントリー: 2BC5 2QLA 3C62
• 分子名称: Soluble cytochrome b562, ZINC ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: 16-helix bundle, interfacial zn-coordination, electron transport, heme, iron, metal-binding, transport
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm : P0ABE7
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49376.06

構造登録者

Tezcan, F.A.,Salgado, E.N.,Lewis, R.A.,Faraone-Mennella, J. (登録日: 2008-02-02, 公開日: 2008-05-06, 最終更新日: 2023-08-30)

主引用文献

Salgado, E.N.,Lewis, R.A.,Faraone-Mennella, J.,Tezcan, F.A.
Metal-mediated self-assembly of protein superstructures: influence of secondary interactions on protein oligomerization and aggregation.
J.Am.Chem.Soc., 130:6082-6084, 2008

PubMed Abstract: We have previously demonstrated that non-self-associating protein building blocks can oligomerize to form discrete supramolecular assemblies under the control of metal coordination. We show here that secondary interactions (salt bridges and hydrogen bonds) can be critical in guiding the metal-induced self-assembly of proteins. Crystallographic and hydrodynamic measurements on appropriately engineered cytochrome cb562 variants pinpoint the importance of a single salt-bridging arginine side chain in determining whether the protein monomers form a discrete Zn-induced tetrameric complex or heterogeneous aggregates. The combined ability to direct PPIs through metal coordination and secondary interactions should provide the specificity required for the construction of complex protein superstructures and the selective control of cellular processes that involve protein-protein association reactions.

PubMed: 18422313
DOI: 10.1021/ja8012177

実験手法

X-RAY DIFFRACTION (1.75 Å)