3C3N

Crystal structure of dihydroorotate dehydrogenase from Trypanosoma cruzi strain Y

3C3N の概要

• エントリーDOI: 10.2210/pdb3c3n/pdb
• 分子名称: Dihydroorotate dehydrogenase, SULFATE ION, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: dhodh, trypanosoma cruzi strain y, dihydroorotate dehydrogenase, pyrd, oxidoreductase
• 由来する生物種: Trypanosoma cruzi
• 細胞内の位置: Cytoplasm (By similarity): Q4D3W2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 138222.10

構造登録者

Pinheiro, M.P.,Iulek, J.,Nonato, M.C. (登録日: 2008-01-28, 公開日: 2008-04-29, 最終更新日: 2024-02-21)

主引用文献

Pinheiro, M.P.,Iulek, J.,Cristina Nonato, M.
Crystal structure of Trypanosoma cruzi dihydroorotate dehydrogenase from Y strain
Biochem.Biophys.Res.Commun., 369:812-817, 2008

PubMed Abstract: Trypanosoma cruzi is the etiological agent of Chagas' disease, a pathogenesis that affects millions of people in Latin America. Here, we report the crystal structure of dihydroorotate dehydrogenase (DHODH) from T. cruzi strain Y solved at 2.2A resolution. DHODH is a flavin mononucleotide containing enzyme, which catalyses the oxidation of l-dihydroorotate to orotate, the fourth step and only redox reaction in the de novo biosynthesis of pyrimidine nucleotides. Genetic studies have shown that DHODH is essential for T. cruzi survival, validating the idea that this enzyme can be considered an attractive target for the development of antichagasic drugs. In our work, a detailed analysis of T. cruzi DHODH crystal structure has allowed us to suggest potential sites to be further exploited for the design of highly specific inhibitors through the technology of structure-based drug design.

PubMed: 18302934
DOI: 10.1016/j.bbrc.2008.02.074

実験手法

X-RAY DIFFRACTION (2.2 Å)