3C2T

Evolution of chlorella virus dUTPase

3C2T の概要

• エントリーDOI: 10.2210/pdb3c2t/pdb
• 関連するPDBエントリー: 3C3I
• 分子名称: Deoxyuridine triphosphatase, MAGNESIUM ION, DEOXYURIDINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: dutpase chlorella virus, hydrolase
• 由来する生物種: Paramecium bursaria Chlorella virus IL3A
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30446.67

構造登録者

Yamanishi, M.,Homma, K.,Zhang, Y.,Etten, L.V.J.,Moriyama, H. (登録日: 2008-01-25, 公開日: 2009-02-24, 最終更新日: 2024-02-21)

主引用文献

Homma, K.,Moriyama, H.
Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase.
Acta Crystallogr.,Sect.F, 65:1030-1034, 2009

PubMed Abstract: The 141-amino-acid deoxyuridine triphosphatase (dUTPase) from the algal Chlorella virus IL-3A and its Glu81Ser/Thr84Arg-mutant derivative Mu-22 were crystallized using the hanging-drop vapor-diffusion method at 298 K with polyethylene glycol as the precipitant. An apo IL-3A dUTPase with an amino-terminal T7 epitope tag and a carboxy-terminal histidine tag yielded cubic P2(1)3 crystals with unit-cell parameter a = 106.65 A. In the presence of dUDP, the enzyme produced thin stacked orthorhombic P222 crystals with unit-cell parameters a = 81.0, b = 96.2, c = 132.8 A. T7-histidine-tagged Mu-22 dUTPase formed thin stacked rectangular crystals. Amino-terminal histidine-tagged dUTPases did not crystallize but formed aggregates. Glycyl-seryl-tagged dUTPases yielded cubic P2(1)3 IL-3A crystals with unit-cell parameter a = 105.68 A and hexagonal P6(3) Mu-22 crystals with unit-cell parameters a = 132.07, c = 53.45 A, gamma = 120 degrees . Owing to the Thr84Arg mutation, Mu-22 dUTPase had different monomer-to-monomer interactions to those of IL-3A dUTPase.

PubMed: 19851015
DOI: 10.1107/S1744309109034459

実験手法

X-RAY DIFFRACTION (3 Å)