3C1V

The 1.5 A Crystal structure of Ca2+-bound S100A4

3C1V の概要

• エントリーDOI: 10.2210/pdb3c1v/pdb
• 分子名称: Protein S100-A4, CALCIUM ION (3 entities in total)
• 機能のキーワード: s100a4, ca2+-bound, calcium-bound, calcium binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 52924.88

構造登録者

Gingras, A.R.,Barsukov, I.L. (登録日: 2008-01-24, 公開日: 2008-04-01, 最終更新日: 2023-11-01)

主引用文献

Gingras, A.R.,Basran, J.,Prescott, A.,Kriajevska, M.,Bagshaw, C.R.,Barsukov, I.L.
Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4
Febs Lett., 582:1651-1656, 2008

PubMed Abstract: S100A4 takes part in control of tumour cell migration and contributes to metastatic spread in in vivo models. In the active dimeric Ca(2+)-bound state it interacts with multiple intracellular targets. Conversely, oligomeric forms of S100A4 are linked with the extracellular function of this protein. We report the 1.5A X-ray crystal structure of Ca(2+)-bound S100A4 and use it to identify the residues involved in target recognition and to derive a model of the oligomeric state. We applied stopped-flow analysis of tyrosine fluorescence to derive kinetics of S100A4 activation by Ca(2+) (k(on)=3.5 microM(-1)s(-1), k(off)=20s(-1)).

PubMed: 18435928
DOI: 10.1016/j.febslet.2008.04.017

実験手法

X-RAY DIFFRACTION (1.5 Å)